This is an image depicting active quick-kill molecule Bax (red) located in the protein-modifying compartment of the cell, the Golgi Apparatus, where it's kept safe so it doesn't accidentally kill the cell. The cell's brain, the nucleus, is stained blue.
Credit: Deshmukh Lab, UNC-Chapel Hill
Embryonic stem cells — those revered cells that give rise to every cell type in the body — will swiftly fall on their metaphorical swords for the greater good if they are injured, new research suggests.
Embryonic stem cells are special because they can give rise to any tissue in the body. Learning more about these cells could help researchers make specific cell types. Specifically, the lab is studying how to grow neurons to treat Parkinson's.
Most research on embryonic stem cells has focused on how to grow them into different cell types in the lab, with studies of the cells themselves lacking, the researchers said. Limitations on government funding of human embryonic stem cell labs have also hampered work in this area.
"Despite the huge potential of stem cells for therapeutic use, very few people have actually investigated their basic biology," study researcher Mohanish Deshmukh, of the University of North Carolina at Chapel Hill, said in a statement. "These results could have significant implications from a therapeutic perspective."
Deshmukh and colleagues treated human embryonic stem cells with DNA-damaging drugs to see what happened. Almost all of the cells committed suicide within five hours, suggesting these types of cells are more sensitive to damage than other cell types, which usually take 24 hours to die once they get damaged.
"Mutations that develop in these cells could be catastrophic for the developing organism, so it would make sense for these cells to be rapidly eliminated," Deshmukh said. This quick-draw death switch is only present for a few days in a stem cell's life, though. Once the cells start down paths to become specific body parts, they deactivate their instant-suicide pathway.
The embryonic cells keep this molecular kill switch, called Bax waiting, around for this very purpose, the researchers found. Instead of activating Bax at the end of a long chain reaction, the embryonic stem cells have it ready and waiting in case of DNA damage.
"What these cells do is very clever," said Deshmukh. "They have activated Bax, but they've also parked it in a safe little compartment." When released, Bax shuts down the cell's power plant, the mitochondria, effectively choking off the cell's energy source.
This cell suicide is critical not only for development (without it, we wouldn't have normal fingers and toes), but also to help stop the spread of disease. "Injured" cells with damaged DNA can lead to mutations that could kill an organism. In this way, a damaged cell is dangerous to its fellow embryonic cells. If the organism dies, so do all of its cells.
The study was published today (May 3) in the journal Molecular Cell.