Stem Cells May Save Northern White Rhinos
The northern white rhino Nabiré, a 32-year-old female at the Dvůr Králové Zoo, died on July 27, 2015. Today, there are only three rhinos of this subspecies left.
Credit: Joel Sartore

With only three northern white rhinoceroses left on Earth, conservationists are giving up on traditional breeding efforts and turning to cutting-edge science to save this subspecies.

At a meeting in Vienna from Dec. 3 to Dec. 6, researchers developed a plan to use stem cells to create fertilized rhino embryos, which will be carried by surrogate southern white rhino females.

This past year has been a sad one for northern white rhinos, a rapidly disappearing subspecies destroyed by habitat loss and poaching. There were six northern whites on the planet, all in captivity, in December 2014. That month, the second-to-last male, Angalifu, died at the San Diego Zoo. That left Sudan, a 42-year-old rhino at the Ol Pejeta Conservancy in Kenya, as the only northern white male rhino on Earth. [In Photos: The Last Surviving Northern White Rhinos]

Next to go was 31-year-old Nabiré, a female who died of a ruptured cyst at the Dvůr Králové Zoo in the Czech Republic in July. An infection claimed Nola, a 41-year-old female at the San Diego Zoo, in November.

Now the only three remaining northern whites live at the Ol Pejeta reserve. Sudan still survives, but is too old to mount a female. And the two remaining females, Najin and Fatu, also have health problems that prevent them from reproducing the old-fashioned way.

So scientists plan to collect egg and sperm cells from the last living northern whites and combine them with induced pluripotent stem cells (iPS cells). These are cells taken from the rhino's body and chemically induced to turn back the clock to an earlier developmental phase, when cells are capable of becoming many different types of body tissue. The hope is that scientists can reverse-engineer body cells into sperm and egg cells. Fertilized embryos could then be made by in vitro fertilization (IVF) and transferred into southern white rhinoceroses, the northern white's nearest relative.

But there are complications to this plan: No one has ever successfully completed IVF on a rhino of any species. Every species requires its own cell-culture conditions to mimic the unique environment of the uterus, Barbara Durrant, director of reproductive physiology at the San Diego Zoo Institute for Conservation Research, told Live Science in June. Depending on how long it takes to make the breakthroughs necessary to create rhino embryos in a lab, the species could go extinct before scientists successfully breed new individuals.

One of the researchers at the Vienna meeting was Katsuhiko Hayashi, a scientist at Kyushu University, who successfully bred mice from eggs created from mouse skin cells in 2012. Researchers are now working to transfer this technology from mice to northern whites, according to a statement from the Dvůr Králové Zoo. The Czech zoo is working with San Diego Zoo Global, Tiergarten Schönbrunn in Austria and the Leibniz Institute for Zoo and Wildlife Research in Berlin to lead the project. A white paper on the group's progress is expected next year.

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